DNA Markers
Ensis has developed DNA markers, which can be used to efficiently resolve genetic questions related to the breeding of acacias, pines and eucalypts.
Markers are being used to tag the likely position of genes controlling traits, which then allows the genes to be physically isolated and characterised. Markers are also ideal for examining the relationships among individuals, populations or phylogenetic taxa.
DNA markers have opened new opportunities for testing the identity of progeny arrays from full-sib crosses in advanced breeding programs.
Using DNA markers, Ensis scientists can now screen full-sib pedigrees to identify progeny derived from foreign pollen or selfing.
The effect of different pollination techniques can also been assessed using DNA markers. Emasculation has been shown to reduce the number of selfed progeny in Acacia mangium crosses from 30% to 1%. Such information can be used to improve the efficiency of controlled pollination programs.
The use of DNA markers also opens new opportunities for breeding programs without the expense of controlled pollination. Breeding populations can be established using a limited number of elite trees, allowed to cross pollinate, and male parentage of superior progeny determined using microsatellite markers. Individuals could then be selected for the next generation, ensuring the maximum number of fathers were represented.
Application of DNA markers for tropical acacias
Ensis is a world leader in the development and application of DNA markers for tropical acacias. Our team has developed the first RFLP and microsatellite markers for Acacia mangium, and has extensive experience in the use of these markers to improve the efficiency of breeding programs.
Major research achievements include identification of differences in levels of genetic variation in natural populations of A. mangium that can be utilised in breeding programs; and findings of significant differences in the level of outcrossing in natural populations and seed orchards of A. mangium. Populations vary from zero to 100% outcrossing.
Further research has demonstrated significant inbreeding depression in growth of A. mangium. Considerable gains in plantation production can therefore be made by ensuring seed is sourced from well designed, highly outcrossing seed orchards. Studies of genetic variation and mating systems have also been published for the commercially important tropical acacias A. aulacocarpa, A. crassicarpa and A. auriculiformis.
Other research achievements include development of the first genetic linkage map for Acacia mangium and location of quantitative trait loci for resistance to the phyllode rust, Atelocauda digitata.
Pricing
No obligation quotes are available upon request
For further information please contact:
Charlie Bell
Ensis Genetics
Ph: +61 2 6281 8324
Fax: +61 2 6281 8312
Email: